ABSTRACT
ABSTRACT Introduction: The traditional lower extremity muscle strength training consists mainly of resistance training, where training intensity is gradually increased, targeting strength gain. Objective: Study the effect of different vibration frequencies on muscle strength training of tennis players' knee joints. Methods: Using PHYSIO-PLATE vibration training platform, tennis players of a Beijing team were subjected to different frequencies of strength training with vibrational stimulation; after eight weeks of systematic strength training, the vibration frequencies were 30Hz and 45Hz, with amplitude of 7mm. Results: After the experiment, the relative peak torque and total work of the knee extensor muscles in subjects in groups I and II were significantly improved (P<0.05), generating a significant increase in rapid maximal power start. Conclusion: The vibrational stimulation addition to muscle strength training can effectively enhance its effect, including characteristics such as maximal strength, rapid strength, and muscular endurance with a relatively small load. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O método tradicional de treinamento de força muscular das extremidades inferiores consiste principalmente no treinamento de resistência, onde é aumentada gradualmente a intensidade do treinamento visando o ganho de força. Objetivo: Estudar o efeito de diferentes frequências de vibração sobre o treino de força muscular nas articulações do joelho de tenistas. Métodos: Usando a plataforma de treinamento de vibração PHYSIO-PLATE, os tenistas de uma equipe de Pequim foram submetidos a diferentes frequências de treinamento de força com estimulação vibratória, após oito semanas de treinamento de força sistemático, as frequências de vibração foram de 30Hz e 45Hz, com amplitude de 7mm. Resultados: Após o experimento, o torque de pico relativo e o trabalho total dos músculos extensores do joelho nos indivíduos dos grupos I e II foram significativamente aprimorados (P<0,05), gerando um significativo aumento na potência máxima rápida de arranque. Conclusão: A adição do estímulo vibratório ao treinamento de força muscular pode efetivamente melhorar seu efeito, incluindo características como a força máxima, força rápida e resistência muscular com uma carga relativamente pequena. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: El método tradicional de entrenamiento de la fuerza muscular de las extremidades inferiores consiste principalmente en el entrenamiento de la resistencia, donde la intensidad del entrenamiento se incrementa gradualmente con el objetivo de ganar fuerza. Objetivo: Estudiar el efecto de diferentes frecuencias de vibración en el entrenamiento de la fuerza muscular de las articulaciones de la rodilla de los tenistas. Métodos: Utilizando la plataforma de entrenamiento de vibración PHYSIO-PLATE, los jugadores de tenis de un equipo de Pekín fueron sometidos a diferentes frecuencias de entrenamiento de fuerza con estimulación de vibración, después de ocho semanas de entrenamiento de fuerza sistemático, las frecuencias de vibración fueron 30Hz y 45Hz, con amplitud de 7mm. Resultados: Después del experimento, el par máximo relativo y el trabajo total de los músculos extensores de la rodilla en los sujetos de los grupos I y II mejoraron significativamente (P<0,05), generando un aumento significativo en el inicio de la potencia máxima rápida. Conclusión: La adición de la estimulación vibratoria al entrenamiento de la fuerza muscular puede mejorar eficazmente su efecto, incluyendo características como la fuerza máxima, la fuerza rápida y la resistencia muscular con una carga relativamente pequeña. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
ABSTRACT
@#[Abstract] Objective: To investigate the effect of double oxidase 2 (DUOX2) on the sensitivity of colorectal cancer (CRC) cells to 5-fluorouracil (5-FU). Methods: CRC cell lines DLD-1, SW480, HCT116, SW620 and normal intestinal epithelial cell line NCM460 were selected, and the expression of DUOX2 in these cell lines were detected by qPCR. DUOX2 expression in HT-29 and HCT116 cells was stably knocked down by lentivirus infection technique. The knockdown efficiency was detected by qPCR and WB. Cells in sh-Control and sh-DUOX2 groups were treated with 5-FU at different concentrations (0, 5, 10, 20, 40, 80, 120 μg/ml). The effects of 5-FU on cell proliferation, apoptosis and cell cycle were detected by CCK-8 method and flow cytometry. HT29 cell transplanted xenograft model in nude mice was constructed to observe the effect of DUOX2 gene on the treatment efficacy of 5-FU. Results: the expression level of DUOX2 mRNA in CRC cells was significantly higher than that in NCM460 cells (P<0.05 or P<0.01). Compared with sh-Control group, the mRNA and protein expressions of DUOX2 in sh-DUOX2 group were significantly decreased (all P<0.01); the sensitivity of cells to 5-FU was enhanced, the apoptosis rate and the ratio of cells at G0/G1 phase were significantly increased (all P<0.01), and the ratio of cells at G2 and S phase was significantly decreased (all P<0.01). There was no significant difference in tumor volume and mass between sh-Control group and sh-DUOX2 group without 5-FU treatment (all P>0.05), but the volume and mass of transplanted tumor in sh-DUOX2+5-FU group after 5-FU treatment was significantly lower than that in sh-Control+5-FU group (all P<0.01). Conclusion: The sensitivity of CRC cells to 5-FU can be significantly enhanced by knocking down DUOX2 gene.
ABSTRACT
@#Objective: To detect the expression of miR-133a-3p in gastric cancer (GC) tissues and plasma of GC patients, and to investigate its effect on the proliferation of GC cells as well as its correlation toprognosis of GC patients. Methods: 52 cases of cancertissues (non-necrosis part) and corresponding adjacent tissues as well as the pre-operative peripheral blood samples from GC patients, who underwent surgery at Department of General Surgery, the Forth Hospital of Hebei Medical University(Shijiazhuang, China) between May 2012 and May 2013, were collected for this study. The plasma sample (n=35) from healthy donors were obtained during their physical examination. RT-qPCR was adopted to detect the expression of miR-133a-3p in gastric cancer tissues, adjacent tissuesand plasma samples of GC patients and healthy volunteers. The relationships between miR-133a-3p expression and the median DFS as well as clinicopathological parameters were also analyzed. CCK-8 assay was adopted to detect the effect of miR-133a-3p silence or over-expression on proliferation of gastric cancer SGC7901 cells. Results: miR-133a-3p was dramatically decreased in gastric cancer tissues (P<0.01), and its expression was associated with TNM stage, tumor infiltration (T), lynphonode metastasis (N), and vascular tumor thrombus (all P<0.01); miR-133a-3p was significantly increased in the plasma of GC patients (P<0.01), and its expression was associated with TNM stage, lynphonode metastasis (N), and vascular tumor thrombus (all P<0.05). miR-133a-3p expression was positively correlated with serum CA199 level of GC patients (P<0.01). The median DFS of patients with high miR-133a-3pexpression in cancer tissues was significantly longer than that of the patients with low expression(20.8 vs 14.8 months, P<0.05); The median DFS of patients with high plasma miR-133a-3p expression was significantly shorter than that of the patients with low expression (14.4 vs 20.3 months, P<0.05). Over-expression of miR-133a-3p could significantly inhibit the proliferation of gastric cancer SGC7901 cells, while miR-133a-3p silence could significantly promote the proliferation (all P<0.05). Conclusion: miR-133a-3p could significantlyinhibit the proliferation of SGC7901 cells; miR-133a-3p aberrantlyexpressed in gastric cancer tissues and plasma, and obviously correlated with prognosis of gastric cancer patients, which may be used as a potential clinical bio-maker for early diagnosis and treatment as well as the prognosis prediction of gastric cancer.